技术支持_上海生因生物科技有限公司

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技术支持

取样说明

细胞样品

组织样品

血液样品

cell-free样品

RNA提取

建库测序

个性化售后分析

组学图表绘制

技术支持

生因生物引进最新的测序平台包括Illumina Hiseq 2000、Hiseq 4000、Hiseq Xten、PacBio RS II、PacBio Sequel，提供各种RNA、DNA分子的建库测序服务。

DNA建库测序

DNA文库	测序测序	测序仪器	样品需求量	Q20	Q30	周期
renjiyinzuzhongcexu	pe150	hiseq x	≥10ug	90%	85%	20gongzuori
waixianzicexu	pe150	hiseq x	≥10ug	90%	85%	20gongzuori
danxibaojiyinzucexu	pe150	hiseq 4000	≥10ng	95%	90%	20gongzuori
dongzhiwude novocexu	PE150 3-10kbp	HiSeq 4000 pacbio rs ii	≥30ug	95%	90%	30gongzuori
yichuantupugoujian	pe150	hiseq 4000	≥30ug	95%	90%	30gongzuori
quanjiyinzuguanlianfenxi	pe150	hiseq 4000	≥10ug	95%	90%	20gongzuori
hi-czuzhuang	pe150	hiseq 4000	≥20ug	95%	90%	30gongzuori
chip-seq	pe150	hiseq 4000	≥10ng	95%	90%	20gongzuori
hongjiyinzucexu	pe150	hiseq 4000	≥5ug	95%	90%	20gongzuori
xijun/zhenjunde novocexu	3-10kbp	pacbio rs ii	≥10ug	95%	90%	20gongzuori

RNA建库测序

RNA文库	测序测序	测序仪器	样品需求量	Q20	Q30	周期
zhuanluzucexu	pe150	hiseq 4000	≥5ug	90%	80%	20gongzuori
lncrnacexu	pe150	hiseq 4000	≥5ug	90%	80%	20gongzuori
circrnacexu（quxianxingrna）	pe150	hiseq 4000	≥10ug	95%	90%	20gongzuori
small rnacexu	se50	hiseq 2000	≥5ug	95%	90%	20gongzuori
quanzhuanluzucexu	pe150	hiseq 4000	≥5ug	95%	90%	20gongzuori
danxibaozhuanluzucexu	pe150	hiseq 4000	≥10ng	95%	90%	20gongzuori
quanzhangzhuanluzucexu	3-10kbp	pacbio rs ii	≥5ug	95%	90%	20gongzuori
rip-seq	pe150	hiseq 2000	≥10ng	95%	90%	20gongzuori
hongzhuanluzucexu	pe150	hiseq 4000	≥5ug	95%	90%	20gongzuori

PCR产物建库测序

PCR文库	测序测序	测序仪器	样品需求量	Q20	Q30	周期
16s/18s rdna	pe150	hiseq 2000	≥5ug	95%	90%	10gongzuori
pcrkuozengchanwuwenku	pe150	hiseq 2000	≥5ug	95%	90%	10gongzuori

技术平台

HiSeq X

PE150  

1.8Tb/Run    

3天

HiSeq 4000 ;

PE150

1.5Tb/Run

3天

HiSeq 2000

PE100

600Gb/Run

3天

PacBio RS II

3-10kbp

16smart cells

30min

PacBio Sequel

3-10kbp

16smart cells

30min