建库测序
DNA建库测序
|
DNA文库 |
测序测序 |
测序仪器 |
样品需求量 |
Q20 |
Q30 |
周期 |
|
renjiyinzuzhongcexu |
pe150 |
hiseq x |
≥10ug |
90% |
85% |
20gongzuori |
|
waixianzicexu |
pe150 |
hiseq x |
≥10ug |
90% |
85% |
20gongzuori |
|
danxibaojiyinzucexu |
pe150 |
hiseq 4000 |
≥10ng |
95% |
90% |
20gongzuori |
|
dongzhiwude novocexu |
PE150 |
HiSeq 4000 |
≥30ug |
95% |
90% |
30gongzuori |
|
yichuantupugoujian |
pe150 |
hiseq 4000 |
≥30ug |
95% |
90% |
30gongzuori |
|
quanjiyinzuguanlianfenxi |
pe150 |
hiseq 4000 |
≥10ug |
95% |
90% |
20gongzuori |
|
hi-czuzhuang |
pe150 |
hiseq 4000 |
≥20ug |
95% |
90% |
30gongzuori |
|
chip-seq |
pe150 |
hiseq 4000 |
≥10ng |
95% |
90% |
20gongzuori |
|
hongjiyinzucexu |
pe150 |
hiseq 4000 |
≥5ug |
95% |
90% |
20gongzuori |
|
xijun/zhenjunde novocexu |
3-10kbp |
pacbio rs ii |
≥10ug |
95% |
90% |
20gongzuori |
RNA建库测序
|
RNA文库 |
测序测序 |
测序仪器 |
样品需求量 |
Q20 |
Q30 |
周期 |
|
zhuanluzucexu |
pe150 |
hiseq 4000 |
≥5ug |
90% |
80% |
20gongzuori |
|
lncrnacexu |
pe150 |
hiseq 4000 |
≥5ug |
90% |
80% |
20gongzuori |
|
circrnacexu（quxianxingrna） |
pe150 |
hiseq 4000 |
≥10ug |
95% |
90% |
20gongzuori |
|
small rnacexu |
se50 |
hiseq 2000 |
≥5ug |
95% |
90% |
20gongzuori |
|
quanzhuanluzucexu |
pe150 |
hiseq 4000 |
≥5ug |
95% |
90% |
20gongzuori |
|
danxibaozhuanluzucexu |
pe150 |
hiseq 4000 |
≥10ng |
95% |
90% |
20gongzuori |
|
quanzhangzhuanluzucexu |
3-10kbp |
pacbio rs ii |
≥5ug |
95% |
90% |
20gongzuori |
|
rip-seq |
pe150 |
hiseq 2000 |
≥10ng |
95% |
90% |
20gongzuori |
|
hongzhuanluzucexu |
pe150 |
hiseq 4000 |
≥5ug |
95% |
90% |
20gongzuori |
PCR产物建库测序
|
PCR文库 |
测序测序 |
测序仪器 |
样品需求量 |
Q20 |
Q30 |
周期 |
|
16s/18s rdna |
pe150 |
hiseq 2000 |
≥5ug |
95% |
90% |
10gongzuori |
|
pcrkuozengchanwuwenku |
pe150 |
hiseq 2000 |
≥5ug |
95% |
90% |
10gongzuori |
技术平台
|
|
HiSeq X
PE150
1.8Tb/Run ; 3天 |
 |
|
|
HiSeq 4000 PE150 1.5Tb/Run 3天 |
|
|
|
HiSeq 2000 PE100 600Gb/Run 3天 |
|
|
|
PacBio RS II 3-10kbp 16smart cells 30min |
|
|
|
PacBio Sequel 3-10kbp 16smart cells 30min |
|
